DIY - tissue culture and rooting plugs

Discussion in 'D.I.Y.' started by f-fish+plants, Nov 19, 2017.

  1. f-fish+plants
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    f-fish+plants Aquascaper

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    As posted in the buce thread ... I want to get into tissue culture. This is a chime in if you want thread. Hopping it will be more than just my ramblings. - Side note you are responsible for what you do and take from this thread. This is just some random thoughts not to be construed as advice that is fit for purpose. No liability is implied or accepted.
     
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  2. f-fish+plants
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    It all started yesterday when I found some agar agar. First batch agar, water, SA, sugar, fertz --- pinch and a boil. No real process was followed - wing it.
    [​IMG]
    I was quick to make my first batch - but much reading and experimentation is still needed.
    [​IMG]

    BIOTOPE ONE - has been pure inspiration after realizing I had no clue what I was mixing or what was needed. So we start from scratch next time doing explant sterilization as described here http://www.biotopeone.com/nadcc-pool-chemical-as-an-explant-sterilization-tool/ (doubt my idea of H2O2 and bleach baths actually worked. So next time I have some NaDCC at the ready).

    [​IMG]

    Not the one with the added Copper ..

    Later Ferdie
     
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  3. f-fish+plants
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  4. Pierré Schoonraad
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    Pierré Schoonraad Green fingers

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    And the, not so mad, scientist is at it again. I knew there was a new thread comming when I saw your other post. What I have gathered about tissue culture plants is that for optimum growth you need to stimulate the 2 sides of growth, leaves and roots, seperately. The person I spoke to about this was unfortunately very secretive about this and wasn't willing to give any further info about this. But I believe with some searching the info will be found.

    Plant propogation with tissue culture is defnitly the way forward as you need minimum space to get a lot of plants going.

    Good luck. I will defnitly follow closely.

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  5. f-fish+plants
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    Hmm roots I am assuming is the agar and stuff in it. Leaves - would that not be the correct spectrum ... worth more reading for sure.

    Later Ferdie

    BTW I do not have an autoclave (no really I don't) and would not want to do this in the pressure cooker ... reading that the microwave has some value in the process. Well at minimum for the melting part .. http://jcm.asm.org/content/7/4/401.full.pdf
     
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  7. Pierré Schoonraad
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    Pierré Schoonraad Green fingers

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    And fish keeping stays a negotiation game.

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  9. Lightweight
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    Lightweight Green fingers

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    Not related but I use agar agar powder to bind my Homemade shrimp ,koi and fish food. Seems it has multiple functions. Thanks for the info
     
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  10. f-fish+plants
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    How much agar agar do you add to the mix when you do the DIY food, do you not find that it breaks up rather fast once it hits the water?
     
  11. f-fish+plants
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    Light arrived ... - no lens coves so probably not the best plan to try and hang this over our aquarium.

    [​IMG]

    Nice to see they think it will work for tissue culture.

    [​IMG]

    Later Ferdie
     
  12. Lightweight
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    Lightweight Green fingers

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    Hi, actually no , my ratio for shrimp food is 0,4 percent of total weight of pre mix food without water. Only powder food used to pre mix , I have different recipes to vary the diet . Example Monday ,Wednesday and Sunday high protein diet . Hemp seed powder or soya powder 50 percent then spinach or kale powder and moringa powder will add beta gluten . Add water until thick paste put in big syringe and press out strips . Put on baking sheet and bake not more than 50 degrees for 40 min . The koi food different as you want the food to float and mouths bigger. The ration of agar agar is 0,7 percent of total dried weight. The cooking time also longer.
     
  13. f-fish+plants
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    hmm I have a kg or two of morning very fine, putting int some DIY food is a lekker idea. I think the baking bit is the interesting factor here.

    Gotta give this a bash - esp when I see what sinking algae pills cost these days.

    Later Ferdie
     
  14. Lightweight
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    Lightweight Green fingers

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    If you wanna make sure it sinks press a hole in each pellet/pills with a needle before cooking time . The reason for low C is mainly to keep the vitamins intact. As I understand everything over 50 degrees you start loosing a lot of vitamins
     
  15. f-fish+plants
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    And so the digging continues ... what plastic can one not use for sterilization in say a microwave.

    upload_2017-11-21_21-9-56.png

    Source: http://www.plastpro.co.za/stores/pl...ising Laboratory Plastic ware Autoclaving.pdf


    Symbol Code[3] Description Examples
    Plastics (see resin identification code[4]) [5]
    [​IMG][a] #1 PET(E) Polyethylene terephthalate Polyester fibers, soft drink bottles
    [​IMG][a] #2 PEHD or HDPE High-density polyethylene Plastic bottles, plastic bags, trash cans, oil cans, imitation wood
    [​IMG][a] #3 PVC Polyvinyl chloride Window frames, bottles for chemicals, flooring, plumbing pipes
    [​IMG][a] #4 PELD or LDPE Low-density polyethylene Plastic bags, buckets, soap dispenser bottles, milk bottles, plastic tubes
    [​IMG][a] #5 PP Polypropylene Bumpers, car interior trim, industrial fibers, carry-out beverage cups
    [​IMG][a] #6 PS Polystyrene Toys, flower pots, video cassettes, ashtrays, trunks, beverage/food coolers, beer cups, wine and champagne cups, carry-out food containers, Styrofoam
    [​IMG][a] #7 O (OTHER) All other plastics Polycarbonate (PC), polyamide (PA), styrene acrylonitrile (SAN), acrylic plastics/polyacrylonitrile (PAN), bioplastics
    [​IMG] #9 or #ABS[citation needed] Acrylonitrile butadiene styrene Monitor/TV cases, coffee makers, cell phones, most computer plastic, most FFF 3D printed parts that are not bioplastic such as PLA
    [​IMG] PA[citation needed] Polyamide Nylon

    https://en.wikipedia.org/wiki/Recycling_codes

    i.e. most should work.

    Later Ferdie
     
  16. f-fish+plants
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    Ok going to give these containers a spin ... in the microwave that is.

    [​IMG]

    Later Ferdie
     
  17. f-fish+plants
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    [​IMG]

    Pitty but did expect it .... 1 out of 4 did not make the test.
     
  18. f-fish+plants
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    f-fish+plants Aquascaper

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    Sjoe ... at least I can grow something, even if it is a fungus of sorts :D not too concerned where the contamination got introduced .... just glad something will grow in the mix. So by day 6 one should be able to say if you have a good culture or not. Need to check the refrigerated medium see if it is a tissue problem or from the word go when one mixed the agar etc.

    [​IMG]

    [​IMG]

    Later Ferdie
     
  19. f-fish+plants
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  20. Pierré Schoonraad
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    Pierré Schoonraad Green fingers

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    And no mention of a hazmatsuit?

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