anti-biotic experiment

Discussion in 'General Aquatic Talk' started by henk hugo, Jan 26, 2007.

  1. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    Good morning all,

    I have started another experiment on a 50cm tank covered in the cyanobacteria. I took the following readings before starting the antibiotic treatment:

    PH6.5
    Ammonia: 0
    Nitrite: 0
    Nitrates 1.0

    I dosed a 13 gallon tank with a 250mg Erythromycin capsule that I broke open and dissolved in a container of water. Here are some photos of the tank before dosage.

    [​IMG]

    [​IMG]


    I will take photos everyday for the 3 day course and then photos after the water change on day 4.
     
  2. banjo
    Offline

    banjo Algae harvester

    • APSA Member
    40%
    Joined:
    Sep 5, 2006
    Messages:
    104
    Likes Received:
    0
    Trophy Points:
    26
    Location:
    Cape Town, South Africa
    Thanks Henk! Great idea.
     
  3. Andre
    Offline

    Andre Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    2,202
    Likes Received:
    1
    Trophy Points:
    48
    Location:
    Cape Town
    So henk, what made you decide to use the antibiotics?
     
  4. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    *note to self* do not break open capsule with finger nail... pink dye under finger looks like vote mark
     
  5. d3tained
    Offline

    d3tained Algae harvester

    • APSA Member
    40%
    Joined:
    Sep 12, 2006
    Messages:
    905
    Likes Received:
    0
    Trophy Points:
    26
    Location:
    Edenvale Johannesburg
    Good idea but how did you get the cyanobacteria to grow in this tank in teh 1st place?
     
  6. City bowl
    Offline

    City bowl Noob

    • APSA Member
    Joined:
    Oct 8, 2006
    Messages:
    553
    Likes Received:
    0
    Trophy Points:
    0
    Henk is very good at growing algae so he probably has some secret method! O0
     
  7. Anonymous
    Offline

    Anonymous Guest

    Cyano is very easy to grow. Put stem plants in a tank with no nitrogen source and in a week or so you'll have cyano.
     
  8. d3tained
    Offline

    d3tained Algae harvester

    • APSA Member
    40%
    Joined:
    Sep 12, 2006
    Messages:
    905
    Likes Received:
    0
    Trophy Points:
    26
    Location:
    Edenvale Johannesburg
    I see.Thx for info!!
     
  9. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    easy... just ignore the tank for 2 or 3 months
     
  10. GDJ
    Offline

    GDJ Algae harvester

    • APSA Member
    40%
    Joined:
    Jul 7, 2006
    Messages:
    406
    Likes Received:
    0
    Trophy Points:
    26
    Location:
    Cape Town
    So H_H

    this experiment of yours any particular reason you are doing this or are you doing it to see how quickly the antibiotics work???
     
  11. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    i want to see what the effect will be on the bio-filter
     
  12. Cameron
    Offline

    Cameron Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    1,664
    Likes Received:
    4
    Trophy Points:
    48
    Location:
    Johannesburg
    It should'nt affect the filter from what I can remember. You get gram positive and gram negative bacteria, the filter bacteria is gram positive and cyano is gram negative (or vice versa I can't remember the exact details. Erythromycin only affects the cyano and not your filter, apparently.

    I'd be interested in how fast it clears up from the first dose, are you seeing any results yet Henk?
     
  13. Cameron
    Offline

    Cameron Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    1,664
    Likes Received:
    4
    Trophy Points:
    48
    Location:
    Johannesburg
    Just remember to do water changes during this process as the Cyano will definitley affect your ammonia levels.
     
  14. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    Erythromycin is a very broad spectrum anti-biotic so we will see. i will do a water change on day four....
     
  15. Andre
    Offline

    Andre Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    2,202
    Likes Received:
    1
    Trophy Points:
    48
    Location:
    Cape Town
    it would have been interesting to see the orp values before and after dosing the anti-biotics, but let us know how it goes.

    Apparently the antibiotic that you are using is much more effective agains gram positive bacteria, but it will also affect the gram negative bacteria, although it will not be able to bind to it as effectively as to gram positive bacteria due to their cell wall composition.

    Some info on Gram's stain

    http://www.infoplease.com/ce6/sci/A0821504.html
     
  16. Rattail
    Offline

    Rattail Algae harvester

    • APSA Member
    40%
    Joined:
    Aug 29, 2006
    Messages:
    880
    Likes Received:
    0
    Trophy Points:
    26
    Location:
    Cape Town
    The vet told me to use 2 to 3 x 250mg capsules per 200L, and to repeat on day 4. I could see results by day 2, and by day 3 the cyanobacteria was all gone. I repeated the dosage on day 4 but used 2 capsules. It is now a week later, and it has still not returned.

    It will be interesting to see the effect of the increased dosage.
     
  17. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    I call this experiment a failure. There was a slight die off on day 2 but then the spikes in the tank caused more bacteria to grow thus over running the tank and it went in a vicious cycle. I find this very interesting! i think i must try again in a week
     
  18. Cameron
    Offline

    Cameron Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    1,664
    Likes Received:
    4
    Trophy Points:
    48
    Location:
    Johannesburg
    Are you talking of Ammonia spikes? Are you sure you added the correct dose of anti-biotics for your tanks volume?
    Interesting stuff.

    Now you have to try the 4 day blackout :)
     
  19. henk hugo
    Offline

    henk hugo Noob

    • APSA Member
    Joined:
    Aug 7, 2006
    Messages:
    1,395
    Likes Received:
    0
    Trophy Points:
    0
    Location:
    Cape Town, South Africa
    yup cam - all the websites said 200mg per 10G for 3 days.... going to try a black out for 4 days
     
  20. Cameron
    Offline

    Cameron Green fingers

    • APSA Member
    37%
    Joined:
    Jun 28, 2006
    Messages:
    1,664
    Likes Received:
    4
    Trophy Points:
    48
    Location:
    Johannesburg
    The procedure is as follows, if you follow it to the T it will work for you:

    • Do a 40% water change, siphon as much of the cyano out as you can while removing the water.
    • Dose nutrients back into the tank, have a NO3 level of at least 5-10ppm
    • Wrap up the tank with old towels, plastic sheets or whatever
    • It is important that absolutely no light whatsoever enters the tank
    • Have your filters running normally during this process
    • After 4 days (better 5) remove the covers, the cyano will be gone.
    • You have to do another large water change (40-50%), clean out the filter and dose back your nutrients, keeping NO3 at 10ppm
    • It is most important to not open the covers at all during this time, not even for a peek and not even to feed the fish, the fish will be fine, I promise you!
    • ENJOY :)
     

Share This Page